MMLV-RT
Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) is an RNA-dependent DNA polymerase that can be used for cDNA synthesis and subsequent PCR or qPCR in one-step or two-step RT-PCR or RT-qPCR assays.
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Meridian's MMLV-RT
- Highly sensitive, even with low concentrations of template RNA
- Thermostability up to 45°C, for more challenging transcripts
- High-quality, first-strand cDNA ideal for most RT-PCR and RT-qPCR applications
- Lower RNase H activity for high yield
MMLV-RT, MDX044
For cDNA synthesis and subsequent PCR or qPCR in a one-step or two-step assay.
Documents & Resources
Description
MMLV-RT is a high-quality reverse transcriptase that synthesizes a complementary DNA (cDNA) strand from mRNA or total RNA. With higher thermostability and reduced RNase H activity, it can be used for synthesis with long messenger RNA templates with high sensitivity and efficiency and is ideal for detecting low-level target genes.
Specifications
| Description | MMLV-RT (Moloney Murine Leukemia Virus Reverse Transcriptase) is used for cDNA synthesis and subsequent PCR or qPCR in a one-step or two-step assay. |
| Concentration | 100x |
| Appearance | Clear, colorless solution |
| Application | cDNA synthesis, RT-PCR, PCR, two-step RT-qPCR, one-step RT-qPCR |
| Sample type | RNA |
| Presentation | 1 vial |
| Storage | -20 °C |
| Mix stability | See outer label |
| Consistency | ± 0.5 Ct variance between test and reference sample |
| DNA contamination | None detected in PCR amplification with traces overlay with the negative control on E. coli and mouse genomic DNA specific targets. |
| DNase/RNase Contamination | No detectable degradation |
Catalogs & Brochures
Related Products
FAQs: MMLV-RT
I want to generate cDNA from viral RNA with very high secondary structure, can I use MMLV-RT?
For RNA with very high secondary structure we recommend using 55C MMLV-RT (MDX117), this reverse transcriptase can be used at temperatures up to 60°C which improves the cDNA yield from difficult RNA targets that require higher temperature to denature strong secondary structures.
Can I improve the yield when using MMLV-RT?
Avoiding ribonuclease contamination is of primary concern since high quality template RNA is required. You can add RNase Inhibitor (MDX056), alternatively we also have RNase-Tolerant MMLV-RT (MDX043) which includes an RNase inhibitor.
What buffer do you recommend using with MMLV-RT?
If you are going to make cDNA, you will require a buffer that contains 0.2 mM each dATP, dCTP, dGTP & dTTP, 10 mM DTT (dithiothreitol), 25 mM KCl, 3.5 mM MgCl2 and 50 mM Tris–HCl (7.5). However if you want to do RT-qPCR, we recommend 1-Step qPCR Buffer, 4x (MDX034) or Lyo-Ready 1-Step RT-qPCR Buffer (MDX052).
How is MMLV-RT inactivated?
The reverse transcriptase can be inactivated by adding a chelating agent such as EDTA or heating to 70°C or higher for 10 min.
Does MMLV-RT need a primer?
Yes, to initiate reverse transcription, the MMVL-RT reverse transcriptase require a primer to bind to its complementary sequences on the RNA template and serve as a starting point for synthesis of a new strand. For general cDNA synthesis, random hexamer primers and oligo(dT) primers can be used either separately or mixed. For RT-PCR and RT-qPCR gene-specific primers should be used.
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