Lyo-Ready AOF dUTP DNA and RNA/DNA LAMP
Lyo-Ready AOF dUTP DNA LAMP and Lyo-Ready AOF dUTP RNA/DNA LAMP are glycerol-free, ready-to-use, 4x concentrated, fully optimized mixes for isothermal amplification methods such as loop-mediated isothermal amplification (LAMP), designed to speed up assay development time and reduce time to results. The glycerol-free mixes contain optimized excipients compatible with freezing and lyophilization, making them ideal for point-of-care testing (POCT). Made from animal-origin-free (AOF) ingredients, these mixes are specifically designed to prevent carryover contamination, ensuring reproducible results.
Key Product Features
Description
Lyo-Ready AOF dUTP DNA LAMP combines the latest advances in buffer chemistry and reaction enhancers, together with an optimized concentration of strand-displacing polymerase, dNTPs and proprietary lyo-excipients allowing ambient temperature stabilization through lyophilization and Lyo-Ready AOF dUTP RNA/DNA LAMP also contains reverse transcriptase. dUTP and has been added, for fast, sensitive and highly reproducible DNA and RNA target amplification. In order to produce lyophilized ambient-temperature stable assays, specific primers need to be added to the LAMP mixes prior to lyophilization and Magnesium prior to amplification. The mixes do not contain animal-derived components, aligning with evolving regulatory trends toward more sustainable in vitro systems, reducing reliance on animals while offering cost-effective, room-temperature stability through a lyophilization-ready formulation.Key points:
- Ideal for point of care diagnostic platforms.
- Reduce risk of carryover contamination.
- Concentrated 4x master mix.
- Contains all the required excipients for subsequent lyophilization.
Faster time-to-result (TTR) and better reproducibility
Lyo-Ready AOF dUTP DNA LAMP, MDX301
Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and lyo excipients. Perfect for LAMP assays where there is a greater risk of false positives from cross-over contamination.
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Lyo-Ready AOF dUTP RNA/DNA LAMP, MDX302
Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and lyo-compatible excipients. Perfect for LAMP assays where there is a greater risk of false positives.
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FAQs: Lyo-Ready AOF dUTP DNA LAMP
How is isothermal amplification different from qPCR?
The key difference between loop-mediated isothermal amplification (LAMP) and quantitative PCR (qPCR) lies in how DNA amplification is achieved. LAMP operates at a constant temperature, eliminating the need for thermal cycling. In contrast, qPCR requires precise cycling through multiple temperature stages using a thermocycler.
Instead of using high temperatures to denature DNA, LAMP relies on specialized polymerases, such as Bst DNA polymerase, that have strong strand displacement activity. This allows DNA amplification to occur rapidly and efficiently at a single temperature. As a result, LAMP can produce detectable amounts of DNA in under an hour, and sometimes in as little as 10 minutes.
Can LAMP be used for a multiplex assay?
Yes, it is possible to design a multiplex LAMP test using different primers and probes.
Are reaction conditions the same for lyophilized and liquid mixes?
Yes, the Lyo-Ready LAMP and Lyo-Ready RT-LAMP 1-Step Mixes (including Lyo-Ready AOF dUTP DNA LAMP) can be used as a liquid mix or lyophilized and stored for up to 24 months without affecting the sensitivity of the tests.
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