Lyo-Ready AOF dUTP DNA and RNA/DNA qPCR Saliva
Lyo-Ready AOF dUTP DNA qPCR and RNA/DNA qPCR Saliva Mixes are glycerol-free, inhibitor-tolerant formulations optimized for high-throughput qPCR applications. Made from animal-origin-free (AOF) ingredients, these mixes are specifically designed to tolerate/withstand carryover contamination, ensuring reproducible results. They are highly effective with both crude and purified saliva samples, making them versatile and reliable for a variety of diagnostic applications.
Key Product Features
- Highly sensitive detection of RNA/DNA, even in crude whole blood, serum and plasma samples
- Compatible with lyophilization for developing ambient-temperature stable assays
- Incorporates dUTP, making it ideal for high-throughput and multiplex assays
- Animal origin-free and sustainable, designed to reduce environmental impact while aligning with future eco-regulations
Description
Lyo-Ready AOF dUTP DNA qPCR Saliva (MDX332) uses an advanced one-tube formulation, incorporating the latest innovations in buffer chemistry and PCR enhancers, along with an optimized concentration of recombinant antibody-mediated hot-start polymerase, dNTPs (including dUTP), and MgCl2. Lyo-Ready AOF dUTP RNA/DNA qPCR Saliva, (MDX333) also includes reverse transcriptase and RNase inhibitor for comprehensive nucleic acid detection.
Lyo-Ready AOF dUTP DNA/RNA qPCR mixes utilize the dUTP/UDGase system to prevent carryover contamination, minimizing false positives and ensuring accurate results, especially in high-throughput workflows. BSA-free and sustainable, these mixes also align with evolving regulatory trends toward in vitro systems, reducing reliance on animal-sourced reagents while offering cost-effective, room-temperature stability through their lyophilization-ready formulation.
Saliva, a non-invasive and easily accessible diagnostic specimen, is gaining attention for detecting various diseases. Like serum, it contains hormones, antibodies, growth factors, enzymes, and microbes that serve as biomarkers. However, challenges arise when using saliva for molecular diagnostics due to low analyte concentrations and high levels of PCR inhibitors, which require complex sample preparation to purify DNA/RNA. Our inhibitor-tolerant Lyo-Ready mixes enable sensitive RNA/DNA detection, even in crude saliva and sputum samples, ensuring accurate results across a wide range of diagnostic applications.
Key points:
- Supports the development of saliva-based screening assays, such as assays detecting COVID, influenza, RSV and tuberculosis.
- Suitable for singleplex or multiplex assays detecting RNA or DNA pathogens at very low titers from saliva or sputum samples.
- Validated for detection of saliva viruses and pathogens.
- Offers the flexibility to remain as a liquid mix or be lyophilized, creating ambient-temperature stable assays with extended shelf life.
Example Protocol
| Reagent Name | Volume |
|---|---|
| Lyo-Ready™ AOF Direct DNA qPCR Saliva, 4x | 5 μL |
| Primer-Probe Mix, 20x* | 1 μL |
| Water | Up to 10 μL |
Figure 1. Lyo-Ready AOF Direct DNA qPCR Saliva. Combine reagents as shown for a total reaction volume of 20 μL. Briefly vortex and spin down briefly in a microcentrifuge. Include the reaction for 2 minutes at 95°C and then cycle for 5 seconds at 95°C and 20 seconds at 60°C for 45 cycles.
Lyo-Ready AOF dUTP DNA qPCR Saliva, MDX332
An optimized qPCR master mix tolerant to inhibitors in saliva and sputum. Perfect for multiplexing, high throughput assays where there is a greater risk of false positives from cross-over contamination.
Documents & Resources
Lyo-Ready AOF dUTP RNA/DNA qPCR Saliva, MDX333
An optimized RT-qPCR master mix tolerant to inhibitors in saliva and sputum. Perfect for multiplexing, high throughput assays where there is a greater risk of false positives from cross-over contamination.
Documents & Resources
Catalogs & Brochures
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FAQs: qPCR Saliva Test
What does Animal origin–free (AOF) mean?
Does not contain materials derived from eukaryotic animals.
Is UDGase supplied in the mixes?
No, UDGase (Uracil DNA Glycosylase) needs to be added separately where there is a greater risk of false positives from cross-over contamination.
What are the advantages of using saliva over other sample types?
Saliva testing is compliance with painless, non-invasive sample collection from the patient, making it ideal for use with children or patients that will not comply with blood collections or even intrusive nasal swabs. Saliva tests and sputum tests also increase the efficiency of collection while minimizing sample handling and reducing handling errors.
What types of infectious diseases can saliva detect?
Saliva is a complex fluid containing various enzymes, electrolytes, proteins, nucleic acids, antimicrobial constituents, hormones, cytokines, and antibodies. Its composition virtually reflects the entire state of health and disease in a body, and it has the potential of being a diagnostic medium for a broad range of diseases, such as cancer and infectious agents, CMV, HIV, respiratory viruses (avian influenza viruses and coronaviruses) and bacterial infections.
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