Enzymes

We offer a selection of high-quality enzymes help you develop highly sensitive assays. Ready-to-use PCR and high-fidelity PCR enzymes and DNA polymerases have been developed for fast PCR and are designed for superior sensitivity and yield. A combination of the latest advances in buffer chemistry and PCR enhancers, in conjunction with an antibody, aptamer, or chemical-mediated polymerases and DNA polymerases, promotes rapid, consistently accurate detection of DNA and cDNA targets. Glycerol-free options allow room temperature shipping and storage, extended shelf-life, and increased flexibility in sample volume, and isothermal options are available for loop-mediated isothermal amplification (LAMP).

Products (30)

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Name	Details	Application	Catalog	Order a Sample	Sizes	Description	Wet(Liquid)	Air-Dryable	Name	Glycerol-Free	Lyo-Ready	Concentration	High Concentration	Detail	Safety Data Sheet	COA/Test Release	User Guide	Product Handling Guide
Glycerol-Free Thermostable DNA Helicase (HC)	DNA	LAMP, RPA, RCA, SDA	MDX242	Order a Sample	Available in 20 µL (1 µg) or 200 µL (10 µg) aliquots	Glycerol-free, high concentration enzyme used to unwind double-stranded DNA. It operates at up to 65°C, ideal for demanding diagnostic workflows such as NGS library preparation, SNP detection and mutation analysis	Yes	Yes	Requires excipients	Yes	Yes	50 µg/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free Phi29 DNA Polymerase (HC)	DNA	RCA, WGA	MDX240	Order a Sample	Available in 20 µL (2,000 Unit) or 100 µL (10,000 Unit) aliquots	Glycerol-free, high concentration enzyme with exceptional strand displacement and processive synthesis properties, ideal for rolling-circle amplification (RCA).	Yes	Yes	Requires excipients	Yes	Yes	100 U/?L	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free Taq HS High Conc. DNA Polymerase	DNA	PCR, NGS	MDX170	Order a Sample	Available in 0.025 mL (1,000 Units) or 0.5 mL (25,000 Units) aliquots	Glycerol-free, high concentration, high fidelity DNA polymerase with separate dilution and reaction buffer, ideal for NGS library amplification and gene expression. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	50 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free DNA Pol I Klenow Fragment exo- (HC)	DNA	NGS	MDX218	Order a Sample	Available in 50 µL (10,000 Units) or 1 mL (50,000 Units) aliquots	Glycerol-free, high concentration DNA Pol I Klenow Fragment exo-, designed for dA- and dT-tailing for next-generation sequencing (NGS) adapter ligation and DNA sequencing by the Sanger method.	Yes	Yes	Requires excipients	Yes	Yes	20 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free Heat-labile UDGase (HC)	DNA	qPCR	MDX217	Order a Sample	Available in 20 µL (1,000 Units) or 500 µL (25,000 Units) aliquots	Used to eliminate carryover polymerase chain reaction (PCR) products in qPCR and RT-qPCR,by incorporating dUTP in reactions, so residual products from previous PCR amplifications containing dUs can be digested.	Yes	Yes	Air-Dryable,Lyo-Ready,Requires excipients	Yes	Yes	50 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free UDGase (HC)	DNA	qPCR	MDX216	Order a Sample	Available in 20 µL (1,000 Units) or 500 µL (25,000 Units) aliquots	Used to eliminate carryover polymerase chain reaction (PCR) products in qPCR and RT-qPCR,by incorporating dUTP in reactions, so residual products from previous PCR amplifications containing dUs can be digested.	Yes	Yes	Air-Dryable,Lyo-Ready,Requires excipients	Yes	Yes	50 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free DNA Pol I Klenow Fragment (HC)	DNA	NGS	MDX208	Order a Sample	Available in 200 µL (10,000 Units) or 1 mL (50,000 Units) aliquots	Glycerol-free, high concentration DNA polymerase used for generating blunt ends on any duplex DNA molecule, used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	50 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free T4 DNA Polymerase (HC)	DNA	NGS	MDX207	Order a Sample	Available in 50 µL (500 Units) or 500 µL (5,000 Units) aliquots	Glycerol-free, high concentration DNA polymerase used for generating blunt ends on any duplex DNA molecule, used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	20 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free T4 Polynucleotide Kinase (HC)	DNA	NGS	MDX206	Order a Sample	Available in 25 µL (500 Units) or 250 µL (5,000 Units) aliquots	Glycerol-free enzyme used to phosphorylate the ends of both DNA and RNA during labeling and ligation, mainly used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	20 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free HS Tth DNA Polymerase (HC)	DNA, RNA	RT-qPCR	MDX205	Order a Sample	Available in 50 µL (4,000 Unit) or 500 µL (100,000 Unit) aliquots	Glycerol-free, high concentration, thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity and unlike other RT enzymes, it is not associated with RNase H activity. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	200 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free High-Fidelity Pfu (HC)	DNA	PCR	MDX203	Order a Sample	Available in 0.025 mL (500 Units) or 1 mL (20,000 Units) aliquots	Glycerol-free, high concentration, high fidelity DNA polymerase with separate dilution and reaction buffer, ideal for NGS library amplification and gene expression. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	20 U/µL	Yes	View Details		Certifate of Analysis		Product Handling Guide
Glycerol-Free RNase H (HC)	RNA	RT-qPCR	MDX202	Order a Sample	Available in 100 µL (2,500 Unit) or 250 µL (12,500 Unit) aliquots	Glycerol-free, high concentration RNA-specific endonuclease that hydrolyzes the phosphodiester bonds of RNA, when hybridized to DNA. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	50 U/µL	Yes	View Details		Certifate of Analysis		Product Handling Guide
Glycerol-Free T7 RNA Polymerase (HC)	RNA	NASBA	MDX201	Order a Sample	Available in 50,000 Unit or 500,000 Unit aliquots	Glycerol-free, high concentration DNA-dependant RNA Polymerase that requires a T7 promoter sequence, it an ideal enzyme for in-vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	1,000 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Gycerol-free T4 DNA Ligase (HC)	DNA	NGS	MDX200	Order a Sample	Available in 1,000 Unit or 10,000 Unit aliquots	Glycerol-free, high concentration enzyme that efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. Ideal for lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	50 Weiss unit/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
55C MMLV-RT	RNA	RT-qPCR, RT-LAMP	MDX117	Order a Sample	Available in 50 µL (10,000 Units), 200 µL (40,000 Units) or 1 mL (200,000 Units) aliquot	Highly robust, thermostable reverse transcriptase, ideal for cDNA synthesis from RNA with a high secondary structure such as viral RNA genomes.	Yes	Yes	Requires excipients	Yes	Yes	200 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Proteinase K Solution	Proteins	Extraction	MDX055	Order a Sample	Available in 25 mL aliquots	Proteinase K Solution is an enzyme used to digest most proteins in molecular biological techniques.	Yes	No	No	Yes	No	20 mg/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Uracil DNA Glycosylase	DNA	qPCR	MDX054	Order a Sample	Available in 10 mL (10,000 Rxns) aliquots	Used to eliminate carryover polymerase chain reaction (PCR) products in qPCR and RT-qPCR,by incorporating dUTP in reactions, so residual products from previous PCR amplifications containing dUs can be digested.	Yes	No	No	Yes	No	1 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
MMLV-RT	RNA	RT-qPCR	MDX044	Order a Sample	Available in 200 µL (1,000 Rxns) or 2 mL (10,000 Rxns) aliquots	For cDNA synthesis and subsequent PCR or qPCR in a one-step or two-step assay.	Yes	No	No	Yes	No	100x	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
RNase-Tolerant MMLV-RT	RNA	RT-qPCR	MDX043	Order a Sample	Available in 200 µL (1,000 Rxns) or 2 mL (10,000 Rxns) aliquots	For the detection of RNA at very low levels, it can also be used with a broader temperature range, making it ideal for applications such as blood bank or transplant viral testing.	Yes	No	No	Yes	No	100x	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Lyo-Compatible MMLV-RT	RNA	RT-qPCR	MDX042	Order a Sample	Available in 8 µL (1,000 Rxns) or 80 µL (10,000 Rxns) aliquots	Glycerol-free high concentration reverse transcriptase and dilution buffer. Can be used to create 1-step RT-qPCR mixes for subsequent lyophilization.	Yes	Yes	Requires excipients	Yes	Yes	> 165 U/µL	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Taq DNA Polymerase	DNA Polymerase	PCR	MDX001	Order a Sample	Available in 0.5 mL (500 Units) or 10 mL (50,000 Units) aliquots	Very high yield, high-performance polymerase, to give fast, superior amplification, even with challenging templates and in the presence of PCR inhibitors.	Yes	No	No	Yes	No	5 U/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
High Conc. Glycerol-Free Bst	DNA	LAMP	MDX018	Order a Sample	Available in 80 µL or 800 µL aliquots	High concentration glycerol-free DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).	Yes	Yes	Requires excipients	Yes	Yes	100 units/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Aptamer Taq HS (Glycerol-Free)	DNA	PCR	MDX015	Order a Sample	Available in 20 ?L (1,000 Units) or 1 mL (50,000 Units) aliquots	Glycerol-free, high concentration hot-start Taq DNA polymerase, suitable for difficult, multiplex molecular tests that require fast amplification.	Yes	Yes	Requires excipients	Yes	Yes	50 U/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Taq HS Antibody	Others	PCR	MDX014	Order a Sample	Available in 20 µL (1,000 Units) or 250 µL (12,500 Units) aliquots	Mix of anti-Taq monoclonal antibodies designed to block Taq DNA polymerase activity at room temperature. Used in the preparation of hot-start PCR or qPCR master mixes.	Yes	Yes	Requires excipients	Yes	Yes	50 U/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Bst DNA Polymerase	DNA	LAMP	MDX012	Order a Sample	Available in 1 mL or 10 mL aliquots	DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).	Yes	No	No	Yes	No	8 units/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free Taq HS, 50 U/µL	DNA	PCR	MDX011	Order a Sample	Available in 0.02 mL (1,000 Units) or 0.5 mL (25,000 Units) aliquots	Lyophilization-compatible high-performance Taq DNA polymerase, separate Taq antibody and enzyme dilution buffer, suitable for difficult, multiplex tests that require fast amplification, whilst maintaining high specificity and sensitivity.	Yes	Yes	Requires excipients	Yes	Yes	50 U/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis	User Guide	Product Handling Guide
Low DNA Taq HS 5 U/µL	DNA	PCR	MDX009	Order a Sample	Available in 0.1 mL (500 Units) or 10 mL (50,000 Units) aliquots	Highly purified Taq DNA polymerase with low DNA background and stringent chemical hot-start properties, ideal for PCR of low-copy bacterial targets to avoid false-positive amplification, such as in water testing.	Yes	No	No	Yes	No	5 U/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Taq HS DNA Polymerase	DNA	PCR	MDX008	Order a Sample	Available in 0.1 mL (500 Units) or 10 mL (50,000 Units) aliquots	Very high yield, high-performance, antibody-mediated hot-start polymerase, to give fast, superior amplification, even with challenging templates and in the presence of PCR inhibitors.	Yes	No	No	Yes	No	5 U/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
High-Fidelity Pfu	DNA	PCR, NGS	MDX003	Order a Sample	Available in 2 mL (500 Units) or 10 mL (25,000 Units) aliquots	Thermostable high fidelity Hot Start DNA polymerase, generates blunt-ended amplicons up to 5 kb in length making it ideal for high yields in NGS library amplification.	Yes	No	No	Yes	No	2 U/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide
Glycerol-Free DNA Helicase (HC)	DNA	LAMP, RPA, RCA, SDA	MDX241	Order a Sample	Available in 20 ?L (1 µg) or 200 ?L (10 µg) aliquots	Glycerol-free, high concentration enzyme used to unwind double-stranded DNA with the help of an accessory protein, allowing you to deliver reliable results with the added capability for lyophilization. MutL	Yes	Yes	Requires excipients	Yes	Yes	50 µg/mL	Yes	View Details	Safety Data Sheet	Certifate of Analysis		Product Handling Guide


Title
Specimen Type
Application
Catalog
Sizes
Wet(Liquid)
Air-Dryable
Glycerol-Free
Lyo-Ready
Concentration
High Concentration
Description






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Ready-to-Use PCR and High-Fidelity PCR Enzymes

Meridian also as a selection of reverse transcriptases for cDNA synthesis, including a reverse transcriptase for dealing with RNA that contains high amounts of secondary structure, such as RNA viruses and reverse transcriptase for dealing with crude samples, particularly in RT-PCR and RT-qPCR applications for the detection of very low-copy targets. We also have a high concentration lyophilization ready option, for incorporation into lyophilized RT-qPCR assays for room temperature shipping and storage, extending shelf-life and increased flexibility in sample volume.

We also have RNases and DNases free Proteinase K that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines and it is especially suitable for isolating qPCR and RT-qPCR templates.

Core Enzymes for Nucleic Acid Processes

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Meridian offers diagnostic manufacturers the complete solution with over 3,000 antigens and antibodies covering more than 500 diseases and an extensive range of specialty qPCR/RT-qPCR master mixes and molecular enzymes all manufactured under ISO13485 regulations.

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Catalogs & Brochures

Master Mixes for Molecular Ambient-Temperature Stable Assays

Master Mixes for Molecular Ambient-Temperature Stable Assays

Air-Dryable Mixes

Air-Dryable Mixes

High-performing DNA polymerases Catalog

High-performing DNA polymerases Catalog

A Comprehensive Guide to Essential Reagents for High-Throughput Molecular Diagnostic Assays (MDx)

Accelerating Efficiency: A Comprehensive Guide to Essential Reagents for High-Throughput Molecular Diagnostic Assays (MDx)

Guide to High-Performing Enzymes

Guide to High-Performing Enzymes

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Enzymes

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